CoolLed pE-300lite

  • Simple White Light
  • Broad spectrum
  • Affordable through lab consumables budget



CoolLED’s pE-300lite is a simple white light LED illumination system that offers intense, broad-spectrum LED illumination for fluorescence microscopy. The system is perfect for hospital and regular research laboratory environments and is affordable within consumables budgets.
This mercury-free alternative is safer, more controllable and precise than conventional bulb illumination options. Spectral coverage is from the UV (DAPI excitation) to the Red region (Cy5 excitation).

LED technology is quickly evolving, and since 2017 our new generation has double the irradiance at the sample plane, and for even higher irradiance is available as a Direct Fit model (which also reduces the cost to buy and run thanks to fewer consumables). With ACT Label certification, it’s even an environmentally friendly option for illumination.


The pE-300lite is a simple white light LED microscope illuminator. It provides a broad spectrum of illumination, covering the excitation bands of common fluorophores such as DAPI, CFP, Aqua, FITC, TRITC, TxRed, Cy5 and many more. Operation is by a remote manual control pod with instant on/off and control of irradiance from 0-100%.

It is now possible to view and image your samples brighter than ever

As part of the award winning pE-300 Series, the enhanced pE-300lite now delivers double the irradiance at the microscope sample plane.

1907005-pE-300 Series images

Convallaria sample captured using Photometrics Prime camera with 10 ms exposure.

With the new enhanced pE-300lite, this simple white light, at full power uses under 60 Watts. That is almost a quadruple efficiency increase, and compares to other LED technology which uses 120 to 350 Watts. This leap forward in technology makes LEDs more attractive when compared to old Mercury or Metal Halide technology, with no mercury to dispose of and lower energy costs due to low power consumption and the precise control offered. Where “green” funding is available, the reduction in institutional energy usage improves the return on investment.
With a long lifetime and a comprehensive range of microscope adaptors, the pE-300lite can be fitted to most current and older microscopes and operate for many years without aligning or replacing bulbs. The result is a safe, convenient illumination system without any additional operating costs.

Direct fit or liquid light guide

There are two pE-300lite configuration options: direct fit or liquid light guide. The Direct Fit variant delivers higher irradiance and reduces the cost to buy and run thanks to fewer consumables. As a replacement to a metal halide source on a confocal microscope, the Liquid Light Guide model is particularly suitable for pre-aligning samples before scanning commences. In this way, photo bleaching of the sample can be minimised when LED illumination is used. More information on the available variants can be found under specifications.

Award winning technology

CoolLED’s pE-300 Series was the 2017 winner of the “Go Beyond Award” for “Excellence in sustainability in laboratory and other high technology facilities”. The International Institute for Sustainable Laboratories (I2SL) runs a unique awards program honouring organisations, individuals, products, and projects that are advancing sustainable, high-performance facilities.

The pE-300lite offers:-

  • Simple to Buy
    • Works with everyday fluorophores/stains
    • Affordable through lab consumables budget
    • Broad spectrum - covers UV (DAPI) to Red region (Cy5)
    • Specify for  existing single and multi-band filter sets – no need to buy new filters
  • Simple to Fit
    • Direct fit to the microscope in seconds
    • Wide range of microscope adaptors – fits most microscopes
    • No alignment, a once only adjustment
  • Simple to Use
    • Instant on/off  - No shutters required, no warm up or cool down
    • Stable & repeatable – reliable and consistent results
    • Precise intensity control in 1% steps (0-100%) – no ND filters required
    • Excellent uniformity over field of view – fixed and stable, no alignment necessary


Power requirements
100-240 V a.c. 50/60 Hz, 1.4 A

Power consumption

pE-300 Series Light Source At the wall (includes power supply unit consumption)
Standby 2 W 3.3 W
Full white irradiance 46 W 56.4 W


pE-300lite Light Source: 77 mm(w) x 186 mm(d) x 162(h) – Weight 1.4 kg
pE-300lite Control Pod: 88 mm(w) x 125 mm(d) x 37 mm(h) – Weight 0.32 kg
pE-300lite Power Supply: 167 mm(w) x 67 m(d) x 35 mm(h) – Weight 0.62 kg
pE-Universal Collimator: 44 mm(w) x 86 mm(d) x 44mm(h) – Weight 0.17 kg

Environment and Safety

  • Mercury-free and laser-free
  • Energy Efficient: 80% less power
  • Long lifetime
  • No bulb replacements
  • Reduced risk of eye damage
  • Quiet operation
  • No special disposal regulations or issues


Industry-leading 36 months.