1625萬畫素 高解析度 高感光度之單色CCD
Nikon manufactures CMOS sensors and imaging technologies for professional D-SLR cameras, and they have now optimized for microscopy.
Detects even faint fluorescent signals
7.3µm pixels, high quantum efficiency, and very low read noise allow the DS-Qi2 to even read faint fluorescent signals.
Indian Muntjac Deer Skin Fibroblast Cells, Cytoskeletal F-actin labeled with Alexa Fluor 488 Sample courtesy of: Michael Davidson and Florida State University
Reliable quantitative analysis made possible
With a linearity error of ±1%, the DS-Qi2 is a superb tool for measuring intensities in fluorescence samples, including time-based intensity measurement and ratiometric measurement.
※High frame rate
Fast focusing, even with fluorescent images
Both 2.2 electrons read noise coupled with a large full-well capacity and 0.6 electrons dark current allow the acquisition of fluorescence images with very little noise.
Acquires dim fluorescent signals with ultra-low noise
|LLC-PK1 cells expressing GFP-EB3 tubulin Low noise and large linear full well capacity allows the acquisition of large dynamic range in a single capture. A captured image is shown intensity scaled to show both the brightest and dimmest areas. Sample courtesy of: Michael Davidson, National High Magnetic Field Laboratory, Florida State University|
2.2 electrons read noise, and this, coupled with a large full-well capacity, allows the acquisition of fluorescence images with very little noise and a very high dynamic range.
Fluorescent time-lapse imaging through integration with NIS-Elements software
With a large field of view and pixel density, and low noise, the DS-Qi2 is ideal for time-resolved imaging applications.
Time-lapse images (every 1 second) of LLC-PK1 cells with GFP-EB3 tubulin. Each image represents the maximum intensity projection of the timelapse, allowing visualization of the end-binding protein located on the microtubule plus-ends, and allowing tracing of the microtubule path. DS-Qi2 captures an extremely large field of view, but still represents very fine details as demonstrated in this cropped timelapse sequence from a large FOV image. Objective: CFI Plan Apochromat λ 60x oil / NA: 1.4) Sample courtesy of: Michael Davidson, National High Magnetic Field Laboratory, Florida State University
|Image sensor||Nikon FX-format, Monochrome CMOS senser / Size: 36.0x23.9mm
Effective 16.25 megapixels
|Recordable pixels||4908x3264 pixel (full-pixel), 1636x1088 pixel (3x3 pixel averaging)|
|Cooling method||Electronic cooling|
(recommended exposure index)
|Standard: equivalent to ISO 800 (Selectable from ISO800 to 51200 equivalent)|
|Full well Capacity||60000e- (typ.)|
|Readout noise||2.2e- (typ.)|
|Dark current||0.6e-/p/s (Ta=25°C) (typ.)|
|Live display mode||Full-pixel 4908x3264 pixel (max 6fps) / 3x3 pixel averaging 1636x1088 (max 45fps)|
|Lens mount||F mount|
|Exposure time||100 µsec to 60 sec|
|Image format||BMP, TIFF, JPEG, etc., selectable in NIS-Elements|
|Interface||USB3.0 (computer control connection) x1, External sync input/output x1|
|Power supply||AC100-240V 50/60Hz|
|Dimensions||105 (W) x 134 (D) x 153 (H) mm|
|Operating environment||0-30°C, 80% RH max, 30-40°C, 60% RH max. (without condensation)|