A-picK™ 自動化軟組織/單細胞採集器
!2021全新機型!輕鬆分離指定的細胞!
原廠連結 A-picK™
1.全新搭載PICKCELLS™專用軟體與電動平台,可自動辨識細胞/組織並進行採集
2.適用於活細胞/軟組織的採集,精準度可至單顆細胞。
3.可自動將採集的細胞分離到指定PCR管或多孔盤
4.搭配Olympus IX系列顯微鏡,全機可放入標準生物操作台使用
Nature 論文:Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis
實際影片:
1.全新操作介面 , 數位化控制參數 , 讓採集過程參數可儲存,並容易重現儀器各式數據(真空壓力/做動時間…)
2.新添加PICKCELLS™專用軟體與電動平台,可自動辨識細胞/組織並進行自動採集
3.增加自動釋放細胞機能,可自動將採集的細胞分離到指定的PCR管或多孔盤
4.可處理厚達500um之軟組織樣本,包括腦、肺、肝、脾、腎…
5.搭配可重複使用之耗材(無斷裂或汙染之虞時),是您更經濟有效率的最好選擇
6.單細胞採集再培養 , 成功機會可達95% 以上 , 省去不必要的時間
7.可回推計算細胞貼壁的黏著力(for 細胞力學應用)
8.快速收集所需要的Cell , 讓研究更簡單
9.DCU 針管 從10 um ~ 100um 不同管徑 , 供不同大小樣品使用
10.耗材便宜 , 且可滅菌後 , 重複使用
功能說明
1.Collection and clonal expansion of single cells from adherent cultures
2.Single cell collection and microdissection of native and untreated tissues
3.Collection of regions of interest (ROIs) from fixed (archived) tissues
影片:
UnipicK+最新實機影片
System capabilities and features:
- Automated cell collection and deposition of any adherent and suspension cells without pretreatment or specialized culture dishes
- Automated cell collection and deposition of individual multicellular sphere from 3D cultures without pretreatment or specialized culture dishes
- Automated cell collection and deposition of cells from microfluidic chips without pretreatment or specialized culture dishes
- Automated microdissection from native and fresh frozen tissue sections (up to 500 µm for some tissues) including brain, lung, liver, spleen, kidney, etc.
- Two collection modes: single cell collection and single cell pooling
- Protocol for automatic collection of regions of interest (ROIs) from fixed tissues (e.g. FFPE) using tissue liquefying approaches
- Target size from few micrometers to a few hundred ensuring wide spectrum of collection conditions
- Fully adjustable parameters for pulse duration, vacuum and pressure strengths, speed, and capillary rinsing sequence
Instant calculation of applied force for collection or deposition provided - Single cell adhesion assay permitting comparative and direct adhesion/detachment assays for multiple single cells
- Data storage and retrieval as a tab delimited file
- Image analysis and collection inspection with prior and post image recording of each collection event
- Collections in both bright and dark fields
- Unprecedented flexibility for collection-deposition modes that can be performed from plate to plate, plate to slide, slide to plate, slide to slide, slide to 48-well plate (enables compatibility with 96 plate formats), slide to PCR tubes, and plate to PCR tubes
- Simplified calibration sequence and several default presets for standard collection protocols
- Compatibility with standard biological safety cabinet and cell culture hood
- High viability of collected cells for efficient clonal expansion of collected cells
- Full compatibility with single cell analysis protocols