NEW GENERATION LEDs

The CoolLED pE-300^ultra is a highly controllable fluorescence microscopy Illumination System which offers intense, broad-spectrum LED illumination for imaging most common fluorophores. Fast events can be captured with microsecond switching via multiple TTL inputs, and the ability to mount inline excitation filters allows fast, flexible filter configurations. This, when paired with today’s high-performance multi-band filter sets, facilitates imaging traditionally done via a white Light Source and a filter wheel, with all the benefits of LEDs.

LED technology is quickly evolving, and since 2017 our new generation has double the irradiance at the sample plane, and for even higher irradiance is available as a Direct Fit model (which also reduces the cost to buy and run thanks to fewer consumables). With ACT Label certification, it’s even an environmentally friendly option for illumination.

Description

The pE-300^ultra is the most controllable member of the pE-300 Series. In addition to the many features of the pE-300^lite and the pE-300^white, the system offers precise control over wavelength irradiance and shuttering. Until now these benefits have only been accessible to users of high end, highly flexible LED microscope illuminators such as the CoolLED pE-4000.

It allows adjustment of output in 1% steps, giving precise control. The broad spectrum covers everyday fluorophores: DAPI, CFP, Aqua, FITC, TRITC, TxRed, Cy5 and many more.

 

It is now possible to view and image your samples brighter than ever

As part of the award-winning pE-300 Series, the enhanced pE-300^ultra now delivers double the irradiance at the microscope sample plane.

Convallaria sample captured using Photometrics Prime camera with 10 ms exposure.

With the new enhanced pE-300^ultra, all three channels at full power use just 60 Watts. That is almost a quadruple efficiency increase. This compares to other LED technology that uses 120 to 350 Watts. This leap forward in technology makes LEDs more attractive when compared to old Mercury or Metal Halide technology. Where “green” funding is available, the reduction in institutional energy usage improves the return on investment.

Capturing high-speed events

The pE-300^ultra offers triggering from multiple TTL inputs which, coupled with the ability to mount inline excitation filters, provides microsecond switching of pre-filtered excitation light. This, when paired with today’s high performance multi-band filter sets, facilitates imaging traditionally done using a white Light Source and a filter wheel, with all the benefits of LED – and most excitingly at speeds not previously so affordable. For live cell imaging applications, events can now be captured that might previously have been missed.

Fast multi-channel imaging without pixel shift

Fluorophores can be viewed either individually or in combination, without filter cube changes. This makes the pE-300^ultra ideal for use with multi-band filter sets as the screening process can be simplified when fewer filter cubes are used. Independent control of the three LED channels means that the user can control the level of excitation of each fluorophore independently on a multi-stained sample, potentially removing the need for single-band filter sets altogether.

Sequence runner for multi-channel excitation

It is now possible to run through a sequence of excitation channels using a camera which has only a single TTL-out, thanks to the Sequence Runner mode. The order of each fluorophore to be captured is defined via the simple Control Pod, with the pE-300^ultra Light Source accepting a single TTL output from the experiment set-up’s camera to initiate the step-through of a sequence of excitation channels. This feature is independent of the individual channel TTL inputs on the Light Source.

Direct fit or liquid light guide

There are two pE-300^ultra configuration options: direct fit or liquid light guide. The Direct Fit variant delivers higher irradiance and reduces the cost to buy and run thanks to fewer consumables. As a replacement to a metal halide source on a confocal microscope, the Liquid Light Guide model is particularly suitable for pre-aligning samples before scanning commences. In this way, photo bleaching of the sample can be minimised when LED illumination is used. More information on the available variants can be found under specifications .

Award winning technology

CoolLED’s pE-300 Series was the 2017 winner of the “Go Beyond Award” for “Excellence in sustainability in laboratory and other high technology facilities”. The International Institute for Sustainable Laboratories (I2SL) runs a unique awards program honouring organisations, individuals, products, and projects that are advancing sustainable, high-performance facilities.

Liquid Light Guidewith a fixed 3mm diameter, liquid light guide.  An optional pE-Universal Collimator can be specified in conjunction with a microscope adaptor if required.

The pE-300^ultra offers:-

• Clean bright illumination across the spectrum – excites common fluorescent stains
• Specify for  existing single and multi-band filter sets – no need to buy new filters
• Instant on/off – No shutters required, no warm up or cool down
• Simple to fit, simple to use – no alignment, a once only adjustment
• Stable & repeatable – reliable and consistent results
• Wide range of microscope adaptors – fits most microscopes
• Precise intensity control in 1% steps (0-100%) – no ND filters required
• Excellent uniformity over field of view – fixed and stable, no alignment necessary
• Long lifetime – expected to exceed 25,000 hours of operating time
• Removable inline excitation filter holders – no moving parts
• Individual channel triggering via TTL in microseconds
• Sequence Runner – sequenced excitation from a single TTL-out

Specification

Power requirements

100-240 V a.c. 50/60 Hz, 1.4 A

Power consumption

pE-300 Series	Light Source	At the wall (includes power supply unit consumption)
Standby	2 W	3.3 W
1 band (GYR)	20 W	24.4 W
2 bands (BLU & GYR)	38 W	44.3 W
Full white irradiance (3 bands)	46 W	56.4 W

Dimensions

pE-300ultra Light Source: 77 mm(w) x 186 mm(d) x 162 mm(h) – Weight 1.4 kg
pE-300ultra Control Pod: 88 mm(w) x 125 mm(d) x 37 mm(h) – Weight 0.32 kg
pE-300ultra Power Supply: 167 mm(w) x 67 m(d) x 35 mm(h) – Weight 0.62 kg

pE-Universal Collimator: 44 mm(w) x 86 mm(d) x 44 mm(h) – Weight 0.17 kg

Environment and Safety
LED products are more sustainable and energy efficient than conventional illuminators. CoolLED’s products have the following benefits:

• Mercury-free and laser-free
• Energy Efficient: 80% less power
• Long lifetime
• No bulb replacements
• Reduced risk of eye damage
• Quiet operation
• No special disposal regulations or issues

Warranty

Industry-leading 36 months.