The CMS196M is a cryo-CLEM instrument to enable the full workflow of CLEM.
Apart from maintaining the sample vitrified by means of liquid nitrogen cooling
it provides proven capabilities to safely handle and transfer cryo samples and
image them with optical microscopy while keeping them free of contamination
at all times. The integrated motorised, encoded stage of the latest model, together
with the new LINK software enables high resolution maps of the grid to be
produced quickly and easily. This provides a straight forward method of coordinate
mapping required to locate the same sample in the fluorescence microscope
as well as in the EM.

Although the CMS196M was designed specifically to solve the problem of how to
get vitrified EM grids from the fluorescent microscope into the cryo TEM without
devitrification and contamination through condensation, the stage also had to be
optimized optically to enable the use of high NA lenses.

Up to 3 grids are loaded into a specially designed cassette which is transported
from a plunge freezer in a small sealed container. The container is loaded into a
precooled dry sample loading chamber fitted on an upright fluorescent microscope.
The cassette is then easily loaded onto the viewing bridge using special
manipulation tools.

• Self-contained cryo sample observation system keeping the sample vitrified
  at constant -196°C and avoiding contamination
• Automatic top-up liquid nitrogen top up of the chamber
• Short startup time of only 10 minutes, no mechanical connection between
  sample mount and Dewar, very good stability, low drift in nm range, good
  long-term stability
• Integrated motorised XY stage with optical encoders for high precision
  movement and position readout of better than 1µm
• Fully Automated XY Mapping
• Mounting option for most research grade, upright microscopes from all
  major manufacturers
• Self-aligning magnetic sample cassette system for up to three sample grids
• Support for EM-grids, Auto-Grids (FEI), Membrane carriers (Leica HPM100),
  Bessy grids, planchettes, CryoCapsule from CryoCapCell and custom sample
  holders, no need to handle bare grids
• Sample cassette holder for safe and contamination-free sample loading,
  storage and transfer
• Connects to plunge-freezing and High-Pressure Freezing sample
  preparation protocols
• Integrated sensors for sample temperature monitoring
• Integrated condenser optics for transmitted light brightfield and
  phase contrast
• Fluorescence observation with NA of up to 0.9
• Full day of independent operation with optional 3L autofill LN2 Dewar
• LCD display with button control, standalone joystick operation and full
  software control via USB interface
• Automated heating “bake-out” cycle to drive out moisture
• More than 50 systems proven in the field worldwide, market leader
  in Cryo-CLEM

• Temperature Range  Hold temperature at –196°C
• EM Grids Supported  Standard EM grids and many others including Bessy, Planchette, FEI and Polara
• Motorised XY Resolution  1μm
• Integrated Condenser Optics  For transmitted light brightfield and phase contrast techniques
• Liquid Nitrogen Hold Time  30 minutes
• 3L Dewar (Optional) Hold Time  6 hours
• Microscope Compatibility  Compatible with a wide range of research grade upright microscopes