Temperature and environmental control from < -195°C to 125°C with motorised XY sample manipulators



The MDBCS196 has been developed for the specialized field of cryobiology where
extremely accurate low temperature and position of the sample as well as cooling
rates of up to 5000°C/min are required.

High speed quench cooling is achieved by high speed motorised movement of the
sample from a copper ‘warm post’ onto a pre-cooled block. It is possible to observe
the effect of crystallisation at extremely high cooling rates. This method of high
speed cooling is even faster than submersing the sample in liquid nitrogen and has
enabled cryobiologists to closely investigate vitrification phenomena.

The silver block is of a similar construction to the BCS196 but has its cooling pipes
at 180°C to each other. This reduces the flow of liquid nitrogen needed to cool
the block and minimises any vertical movement of the block due to temperature
change. For seeding a cold spot has been provided on one of the cooling pipes
allowing nucleated freezing to be initiated.

LINK software (included with the stage) enables automatic serpentine scanning of
the entire sample allowing the user to save positions of interest. Add the digital
video capture module to create a image map of the sample and zoom in to areas
of interest.

  • Temperature range < -195°C to 125 °C
  • Controlled heating and cooling rates of 0.01°C to 150°C/min
  • Uncontrolled cooling up to 5000°C/min
  • PC control using LINK software
  • Positional resolution of motors 0.05µm
  • Position repeatability <3um
  • 150 XY coordinates can be stored
  • Easy to fit fine focus attachment Z axis (dependent on microscope)
  • Max 15mm motorised XY travel
  • Definable sample scan area
  • Sample vibration eliminated over entire range of speeds
  • Designed for use with the LINK imaging software
  • Ventilated bottom window to eliminate condensation
  • Novel low profile lid design for rapid lens change
  • Efficient use of liquid nitrogen
  • Stage body size 160mm x 80mm x 24mm
  • Mounts directly on microscope substage using stage-clamps
  • Objective lens working distance 0.1mm to 4.9mm
  • Condenser lens minimum working distance 12.7mm
  • Light aperture 1.3mm Ø for accurate sample temperatures
  • Optional lid and cooling jacket for high magnification lenses