| Fluorescence – enhanced |
Fluorescence – standard |
| Light source |
High energy xenon flash lamp |
Light source |
Dedicated xenon flash lamp |
| Spectral range |
Ex: 230–900 nm
Em: 280–900 nm |
Spectral range |
Ex: 230–900 nm
Em: 280–900 nm |
| Wavelength accuracy |
Ex: <0.5 nm; Em: <0.5 nm |
Wavelength accuracy |
Ex: <1 nm; Em: <2 nm |
| Wavelength reproducibility |
<0.5 nm |
Wavelength reproducibility |
<1 nm |
| Bandwidth |
Adjustable from 5–50 nm |
Bandwidth |
Fixed @ 20 nm |
| Optical mirrors |
50 %, 510, 560, 625 nm built-in; 410, 430, 458, 593, 660 nm user-selectable dichroics |
Optical mirrors |
50 %; 510 nm dichroic |
| Well scanning |
Up to 100 x 100 data points |
Well scanning |
Up to 100 x 100 data points |
| FI (fluorescence intensity) Limit of detection1 |
FI (fluorescence intensity) Limit of detection1 |
| Filter – top |
≤8 amol/well (10 μl; 1,536-well) |
Filter – top |
≤25 amol/well (100 μl; 384 well) |
| Fusion* – top |
≤15 amol/well (10 μl; 1,536-well) |
Fusion – top |
≤35 amol/well (100 μl; 384 well) |
| Mono – top |
≤20 amol/well (10 μl; 1,536-well) |
Mono – top |
≤50 amol/well (100 μl; 384 well) |
| Filter – bottom |
≤180 amol/well (10 μl; 1,536-well) |
Filter – bottom |
≤500 amol/well (200 μl; 96 well) |
| Fusion – bottom |
≤200 amol/well (10 μl; 1,536-well) |
Fusion – bottom |
≤700 amol/well (200 μl; 96 well) |
| Mono – bottom |
≤220 amol/well (10 μl; 1,536-well) |
Mono – bottom |
≤800 amol/well (200 μl; 96 well) |
| FP (fluorescence polarization)2 |
FP (fluorescence polarization)2 |
| Spectral range |
300–850 nm |
Spectral range |
300–850 nm |
| Precision – Filter |
≤1.25 mP |
Precision – Filter |
≤1.5 mP |
| Precision – Fusion |
≤2.0 mP |
Precision – Fusion |
≤2.5 mP |
| Precision – Mono |
≤2.5 mP |
Precision – Mono |
≤3.0 mP |
| TRF (time-resolved fluorescence)3 |
TRF (time-resolved fluorescence)3 |
| Limit of detection – Filter |
≤0.5 amol/well (20 μl; 384-well SV) |
Limit of detection – Filter |
≤4.0 amol/well (100 μl; 384-well) |
| Limit of detection – Fusion |
≤0.6 amol/well (20 μl; 384-well SV) |
Limit of detection – Fusion |
≤6.5 amol/well (100 μl; 384-well) |
| Limit of detection – Mono |
≤0.7 amol/well (20 μl; 384-well SV) |
Limit of detection – Mono |
≤10 amol/well (100 μl; 384-well) |
| Fastest read time |
Fastest read time |
| 384-well plate (FI) |
≤22 sec |
96-well plate (FI) |
≤13 sec |
| 1,536-well plate (FI) |
≤34 sec |
384-well plate (FI) |
≤30 sec |
| Absorbance (enhanced or standard) |
| Light source |
Dedicated xenon flash lamp |
| Spectral range |
200–1,000 nm
OD range 0–4 OD |
| Scan speed (200–1,000 nm) |
≤5 sec |
| Wavelength accuracy |
<0.3 nm |
| Wavelength reproducibility |
≤0.3 nm |
| Wavelength ratio accuracy (260/230) |
<0.08 |
| Wavelength ratio accuracy (260/280) |
<0.07 |
| Precision @ 260 nm |
<0.2 % |
| Accuracy @ 260 nm |
<0.5 % |
| Limit of detection (nucleic acids) |
<1 ng/μl |
| Plate formats for all read modes – enhanced |
Plate formats for all read modes – standard |
| 1-1,536 wells; NanoQuant Plate; Cuvettes; Roboflask® |
1-384 wells; NanoQuant Plate; Cuvettes; Roboflask |
| Luminescence (enhanced or standard) |
| Spectral range |
370–700 nm |
| Limit of detection – Glow4 |
≤225 amol/well (25 μl; 384-well SV) |
| Limit of detection – Flash5 |
≤12 amol/well (55 μl; 384-well) |
| Dynamic range |
>9 orders of magnitude |
| Multi-color luminescence |
38 spectral filters; OD1, OD2, OD3 attenuation filters |
| AlphaScreen (enhanced or standard) |
| Limit of detection |
<100 amol/well bio-LCK-P6; 20 μl
<2.5 ng/ml Omnibeads7; 20 μl |
| Uniformity |
≤3.0 % |
| Z´value |
>0.9 |
| Fastest read times8 |
≤2 min (384-well plate)
≤1 min (96-well plate) |
| Gas Control Module (GCM™) |
| Adjustable concentration range – CO2 |
0.04–10 % (vol.) |
| Adjustable concentration range – O2 |
0.1–21 % (vol.) |
| Concentration accuracy – CO2 |
<1 % (vol.) |
| Concentration accuracy – O2 |
<0.5 % (vol.) |
| Reagent injectors |
| Syringe sizes |
0.5 ml; 1 ml |
| Pump speed |
100–300 μl/sec |
| Injection volume |
5–2,500 μl; step size: 1 μl |
| Dead volume |
≤100 μl |
| Injection accuracy and precision |
≤0.5 % at 450 μl |
| Temperature control |
Ambient +3 °C up to 42 °C |
| Uniformity |
<0.5 °C |
| Shaking |
| Linear, orbital, double-orbital; variable amplitudes and frequencies |
