| DISPENSING FEATURES |
|
 |
 |
| T8+ Dispensehead Cassette |
D4+ Dispensehead Cassette |
| Fluid type |
DMSO |
Aqueous +
surfactant |
Aqueous +
surfactant +
glycerol |
Master mix |
DMSO,
Aqueous + surfactant,
Aqueous + surfactant + glycerol,
Master mix |
| Use for |
Titration,
Low volume dispensing and normalization of master mixes, aqueous fluids (+ surfactants) and DMSO |
| Dispenseheads per cassette |
8 |
|
|
|
4 |
| Minimum dispense volume |
13 pl |
11 pl |
12 pl |
11 pl |
1 nl |
Minimum dispense volume
(Total dispense volume >10 μl) |
1 nl |
1 nl |
1 nl |
1 nl |
1 nl |
| Maximum dispense volume per well |
10 μl |
10 μl |
10 μl |
10 μl |
10 μl |
| Minimum fill volume |
2 μl |
4 μl |
4 μl |
4 μl |
50 μl |
| CV |
< 8%* |
< 8%** |
< 8%** |
< 8%*** |
< 8%**** |
| * for volumes >100 pl using 100 % DMSO |
| ** for volumes >100 pl using aqueous test fluid containing 0.1 % Brij™-35 |
| *** for volumes >100 pl using Master mixes included in the fluid class list |
| **** for volumes >1 nl using test fluids listed above and Master mixes included in the fluid class list |
| FLUID REQUIREMENTS |
|
|
|
| T8+ Dispensehead Cassette |
D4+ Dispensehead Cassette |
| Maximum total dispense volume |
10 μl |
20 μl |
200 μl |
| Small molecules |
Max. 800 Da at 10 mM |
Max. 800 Da at 10 mM |
Max. 800 Da at 10 mM |
| Biomolecules |
300 kDa, 3 mg/ml |
300 kDa, 1 mg/ml |
300 kDa, 0.3 mg/ml |
| 10,000 bp, 3 mg/ml |
10,000 bp, 1 mg/ml |
10,000 bp, 0.3 mg/ml |
| Nanoparticles |
Stable suspension of < 1 μm diameter particles at max. 0.5 % w/v |
| Total buffer components <150 mM |
| FLUID CLASSES |
| Aqueous solutions require the addition of a surfactant to reduce surface tension and enable priming. In addition, the presence or absence of glycerol impacts dispensing – there are separate fluid classes dependent on glycerol content. |
| Fluid class |
Surfactant |
Surfactant concentration in stock solution |
Glycerol |
| a + B |
Brij-35 |
0.1 % |
0-5 % |
| a + B + g |
Brij-35 |
0.1 % |
5-20 % |
| a + TX |
TritonR X-100 |
0.1 % |
0-5 % |
| a + TX + g |
Triton X-100 |
0.1 % |
5-20 % |
| a + Tw |
Tween™ 20 |
0.3 % |
0-5 % |
| a + Tw + g |
Tween 20 |
0.3 % |
5-20 % |
| DIMENSIONS |
| Dimensions |
(W x D x H): 47 x 37 x 23 cm |
| Plate formats |
12–1536 well plates, 6–47 mm plate height |
| Weight |
15 kg |
| Electrical |
100-240 V, 50/60 Hz, 2.6 A |
| Operating Temperature |
20-30 °C (68-86 °F) |
